Figure 1.

LS2 arose and diverged in function from dU2AF⁵⁰ in Drosophila. (A) Phylogenetic tree of LS2, dU2AF⁵⁰, and orthologs from honey bees, mosquitoes, and humans. The non-Drosophila gene sequences are the single most similar genes to both dU2AF⁵⁰ and LS2 in each of the three outgroup genomes. Clades with >90 credibility value are denoted with a black circle. (B) Venn diagram showing the overlap of splice junctions affected by dU2AF⁵⁰, dU2AF³⁸, and LS2 RNAi knockdown. (C) A scatter plot of splice junctions affected by dU2AF⁵⁰ and LS2 RNAi knockdown. Axes represent the log₂ change of splice junction intensity in response to RNAi of the indicated protein.