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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Cancer Res. 2011 Mar 15;71(6):2183–2192. doi: 10.1158/0008-5472.CAN-10-3626

Figure 2. CARMA3 is required for EGF-induced NF-κB activation.

Figure 2

(A) MEF cells from Carma3+/+ or Carma3−/− mice were stimulated with indicated doses of EGF (μM) or TNFα (10 ng/ml). (B) CARMA3-knockout cells were reconstituted with wild type CARMA3 (C3KO-WT) or vector control (C3KO-Vec) were stimulated with indicated doses of EGF or TNFα (10 ng/ml). Nuclear extracts were prepared and then subjected to EMSA using 32P-labeled NF-κB or Oct-1 probe. (D) C3KO-WT or C3KO-Vec cells were stimulated with EGF (100 μM) for indicated time and then cell lysates were subjected to immunoblotting analysis using indicated antibodies. (C) C3KO-WT or C3KO-Vec cells were stimulated with EGF (100 μM) or TNFα (10 ng/ml) for indicated time. Cell lysates were subjected to immunoblotting analysis using indicated antibodies. (D) MEF cells from Bcl10+/− or Bcl10−/− mice were stimulated with indicated concentration of EGF (μM) or TNFα (10 ng/ml) for one hour. Nuclear extracts were prepared from these cells, and then subjected to EMSA using 32P-labeled NF-κB probe.