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. Author manuscript; available in PMC: 2012 Apr 1.

Published in final edited form as: Insect Mol Biol. 2010 Nov 30;20(2):201–213. doi: 10.1111/j.1365-2583.2010.01058.x

Expression of PTTH mRNA after blood feeding in nondiapausing and diapausing females. A: Time-course of PTTH mRNA expression after a blood meal in nondiapausing females. PTTH mRNA increased 7 fold within 2 h and the elevation persisted for at least 24 h. Nondiapausing females used in the experiment were 4-weeks old when offered a blood meal. Nondiapausing females fed a 10% sugar solution were used as controls. Error bars represent standard deviations of three replicates; 15 individuals were sampled in each replicate. Double asterisk and triple asterisks indicate significant differences and very significant differences between the tested group and control group, respectively (*P<0.05, ***P<0.001, one-way ANOVA). B: Length of primary follicles measured in diapausing unfed (D-unfed), diapausing fed (D-fed), nondiapausing unfed (ND-unfed), and nondiapausing fed (ND-fed) groups 5 h after the fed groups received a blood meal. Three follicles were selected randomly for measurement in each female, and 16 females were employed in each group. Bars with different letters are significantly different at P = 0.05, one-way ANOVA. C: Expression of PTTH mRNA in D-unfed, D-fed, ND-unfed, and ND-fed groups 5 h after the fed groups received a blood meal. Error bars represent standard deviations of three replicates; 10 individuals were sampled in each replicate. PTTH mRNA was elevated 4–5 fold after blood feeding in both diapausing and nondiapausing group. All mosquitoes were held at 18°C. Bars with different letters are significantly different at P = 0.05, one-way ANOVA.

Expression of PTTH mRNA after blood feeding in nondiapausing and diapausing females. A: Time-course of PTTH mRNA expression after a blood meal in nondiapausing females. PTTH mRNA increased 7 fold within 2 h and the elevation persisted for at least 24 h. Nondiapausing females used in the experiment were 4-weeks old when offered a blood meal. Nondiapausing females fed a 10% sugar solution were used as controls. Error bars represent standard deviations of three replicates; 15 individuals were sampled in each replicate. Double asterisk and triple asterisks indicate significant differences and very significant differences between the tested group and control group, respectively (*P<0.05, ***P<0.001, one-way ANOVA). B: Length of primary follicles measured in diapausing unfed (D-unfed), diapausing fed (D-fed), nondiapausing unfed (ND-unfed), and nondiapausing fed (ND-fed) groups 5 h after the fed groups received a blood meal. Three follicles were selected randomly for measurement in each female, and 16 females were employed in each group. Bars with different letters are significantly different at P = 0.05, one-way ANOVA. C: Expression of PTTH mRNA in D-unfed, D-fed, ND-unfed, and ND-fed groups 5 h after the fed groups received a blood meal. Error bars represent standard deviations of three replicates; 10 individuals were sampled in each replicate. PTTH mRNA was elevated 4–5 fold after blood feeding in both diapausing and nondiapausing group. All mosquitoes were held at 18°C. Bars with different letters are significantly different at P = 0.05, one-way ANOVA.