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. 2001 Mar 6;98(6):3018–3023. doi: 10.1073/pnas.061305498

Figure 4.

Figure 4

The effects of Smad3 on AR-mediated transcriptional activity. (A) SW480.7 cells were cotransfected with 1 μg of pSG5-AR, 3 μg of MMTV-CAT, and 3 μg of Smad3 expression vectors in the presence (+) or absence (−) of DHT (10−8 M) or TGF-β (10 ng/ml). (B) DU145 cells were cotransfected with 3 μg of Smad3 or Smad3ΔC mutant expression vectors with 1 μg of pSG5-AR and 3 μg of MMTV-CAT, in the presence (+) or absence (−) of DHT (10−8 M) or TGF-β (10 ng/ml). Each CAT activity is presented relative to the transactivation observed in the absence of DHT, and an error bar represents the mean ± SD of four independent experiments.