Figure 7.

AR-induced PSA expression is potentiated by Smad3. (A) Smad3-enhanced androgen/AR-induced PSA mRNA expression. LNCaP cells were transfected with Smad3 and parent vector as indicated for 16 h, followed by DHT treatment for another 16 h. The PSA expression level was determined by Northern blotting. The probe was obtained from exon 1 of the PSA gene and labeled with [α-³²P]dCTP. A β-actin probe was used as a control for equivalent mRNA loading. (B) A model for androgen and TGF-β pathways in AR-mediated PSA transcription.