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. 2011 Feb 11;12(3):276–282. doi: 10.1038/embor.2011.3

Figure 3.

Figure 3

Surface plasmon resonance analysis of laminin α5, β1 and γ1 short-arm interactions. (A) Reducing SDS–PAGE of the α5, β1 and γ1 LN–LEa1–4 fragments used in the analysis. The positions of molecular weight markers (in kD) are indicated. (B,C) Sensorgrams showing binding of β1 to γ1. (D,E) Sensorgrams showing lack of binding of α5 to β1 and γ1 (red) and formation of ternary α5–β1–γ1 complexes (black and grey). The concentrations of soluble analytes are indicated. LE, laminin-type epidermal growth factor-like; LN, laminin N-terminal; PAGE, polyacrylamide gel electrophoresis; WT, wild type.