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pPPIs detected in the AMPv2 screen. (A) Selection of diploid cells on DS media supplemented with adenine and histidine. (B) Successful mating (His3, Ade2 positive) was selected on IS-0 media without met (right) and IS-500 with 500 μM met (left). (C) Representative image of the β-galactosidase assay performed on yeast grown on nitrocellulose-overlaid agar media. Positive pairs were picked by hand, re-arrayed and tested for the LacZ marker. Plate size for (A–C) was 125 mm × 85 mm.
