Figure 1.

Tissue expression pattern of zebrafish fxyd isoforms and structure of zfxyd11 variants. (A) Total RNA isolated from various tissues of adult zebrafish were subjected to RT-PCR amplification by using specific primers for the indicated zebrafish fxyd isoforms and ribosomal protein L9 (rpl9). (B) Amino acid sequences of zFxyd11a and zFxyd11b. The putative signal sequences and the transmembrane (TM) domains are indicated by box and horizontal, respectively. The FXYD motifs are highlighted. (C) Schematic representation of alternative splicing of the zfxyd11 gene. Exons are indicated by boxes, and introns and flanking regions are indicated by horizontal lines. The coding and untranslated regions are indicated by dark and open boxes, respectively. zFxyd11b is generated through alternative use of the stop codon in intron 6. (D) Exon-intron organization and boundary sequence of zfxyd11a. The splice donor and acceptor sequences are bold, and the stop codon of zfxyd11b is indicated by the box.