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. 2010 Aug 23;1:129. doi: 10.3389/fphys.2010.00129

Figure 4.

Figure 4

Localization of zFxyd11 in NaK-MRCs of the gill of adult zebrafish. Immunofluorescence staining was performed on cryosections of the gill of adult zebrafish with anti-Na+–K+-ATPase antiserum [red in (B), (C), (E)] along with either anti-zFxyd11 antiserum [green in (A), (C), (D)] or with preimmune serum [green in (F)]. Nuclei were stained with Hoechst 33342 (blue). High magnification images of zFxyd11-positive cells in the boxed areas in (A,B) are shown in (D,E), respectively. Scale bars in (A–C), 50 μm; in (D,E), 5 μm; in (F), 100 μm.