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. 2010 Nov 26;1:155. doi: 10.3389/fphys.2010.00155

Figure 3.

Figure 3

Analysis of the functional stoichiometry of rClC-K1 channels by non-stationary noise analysis (A) and single channel recordings (B–D). (A) Non-stationary noise analysis of WT rClC-K1 currents in the absence of barttin obtained from whole-cell patch clamp recordings of heterologously expressed channels in mammalian tsA201 cells. Mean currents (upper traces) and variances (middle trace) upon a voltage step that elicits biphasic responses result in a loop-like dependence when displayed in a plot of variances versus mean current amplitudes (lower panel). (B–D) Inside–out patch clamp recordings of mutant V166E rClC-K1 under nearly symmetrical chloride concentration (pipette solution: 150 mM; bathing solution: 124 mM). (B) Single channel recordings in presence of barttin at +45 and +95 mV show fast protopore gating with two equally spaced conductance states. (C) Amplitude histogram from bursts of registration at +95 mV. (D) Probabilities of the three current levels, closed, open 1 and open 2 at +95 mV (bars). Closed circles denote the predicted values for binomially distributed states. (modified from Fischer et al., 2010).