Table 1.
Biophysical and pharmacological characteristics of outward voltage-dependent K+ currents in mononuclear phagocytes.
| Biophysical parameters | Pharmacology | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell type | Origin | Threshold | V1/2 | Conductance | CI | C-type | TEA | 4-AP | CTX | MgTx | References |
| Kv1.3 | −40 | −35 | 13 | + | 250 | 10 | 0.2 | 3 | 110 | Grissmer et al. (1994), http://www.iuphar-db.org/iuphar-ic/KV/KV13.pdf | |
| Kv1.5 | −40 | 14 | 8 | – | >5000 | 330 | 0.3 | – | – | Grissmer et al. (1994), http://www.iuphar-db.org/iuphar-ic/KV/KV15.pdf | |
| Macrophages | Spleen | 15/−21 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | Gallin (1981) |
| Peritoneal | −50 | n.d. | n.d. | n.d. | 450 | 10 | 1 | n.d. | n.d. | Ypey and Clapham (1984) | |
| Alveolar | −40 | −18 | n.d. | + | 561 | 1 | 0.3/4 | n.d. | 160 | Nelson et al. (1990), Mackenzie et al. (2003), Park et al. (2006) | |
| Blood | n.d. | −20 | n.d. | + | 535 | n.d. | 4 | n.d. | n.d. | Nelson et al. (1992) | |
| Bone marrow | −30 | −11/−16 | n.d. | + | 365 | n.d. | n.d. | n.d. | 50 | Vicente et al. (2003, 2006) | |
| Microglia | Brain | −40 | −10/−27 | 8/17 | + | 400/600 | 5/10 | 0.27/5 | 1/50 | 1/10 | Eder et al. (1995, 1998), Pyo et al. (1997), Jou et al. (1998), Kotecha and Schlichter (1999), Cayabyab et al. (2000), Schilling et al. (2000), Chung et al. (2001), Khanna et al. (2001), Fordyce et al. (2005), Pannasch et al. (2006), Menteyne et al. (2009) |
| Dendritic | Spleen | −38 | n.d. | n.d. | + | n.d. | n.d. | n.d. | n.d. | n.d. | Fischer and Eder (1995) |
| Blood | −38 | 10/−25 | n.d. | + | 127 | 12 | n.d. | 3/100 | 40 | Mullen et al. (2006), Zsiros et al. (2009) | |
| Bone marrow | −30 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | 100 | Matzner et al. (2008), Xuan et al. (2009) | |
| THP-1 | Monocytes | −40 | −20 | 20 | n.d. | 300/600 | 12.5 | 0.1 | 1.7 | n.d. | Kim et al. (1996) |
| Raw | Macrophages | −30 | 6.3/−6.8 | n.d. | + | 400/1740 | n.d. | n.d. | n.d. | 319/772 | Vicente et al. (2006), Villalonga et al. (2007) |
Values from heterologously expressed mKv1.3 and hKv1.5 in mammalian cells are indicated for comparison. Indicated with /, values from several studies including control and/or activated cells are mentioned. See text for details. Pharmacological parameters are mostly shown as Ki. However, in some cases, the effective range of the blocker concentration from different studies is also indicated. Threshold and V1/2 units are in mV, conductance in pS. CI, presence (+) or absence (−) of cumulative inactivation; C-type, c-type inactivation in ms. TEA and 4-AP in mM. CTX, charybdoxin in nM. MgTX, margatoxin in pM with the exception of brain microglia in nM. n.d., not determined.