Figure 2.

${\text{K}}_{\text{ATP}}^{+}$ channel involvement in the hyperpolarization produced by FSH and forskolin on the Sertoli cell membrane. Pre-treatment with tolbutamide inhibited the rapid hyperpolarization response without affecting the depolarization phase, register of a representative cell (A). Changes produced by tolbutamide on the FSH response (B). Topical application of forskolin (100 nM) produced hyperpolarization on Sertoli cell membrane (C). Comparison of the FSH and forskolin action on Sertoli cell membrane potential (D). **p < 0.001; n = 5 in each treatment (FSH or FSH plus tolbutamide) from five separated experiments.