Figure 5.

Inhibition of TG II abolishes contraction to NE in aortic and vena cava tissues. (A–D) Effect of vehicle (open symbol) and cystamine (0.01–1 mM) on contraction in the vena cava and aorta. NE-induced contraction was eventually abolished in the aorta (A) and vena cava (B). Contraction to KCI was attenuated, but not abolished in both the aorta (C) and vena cava (D). Cys = Cystamine * indicates statistical difference (p <0.05) from vehicle-treated values. Points and vertical lines represent means ± SEM for the number of animals in parentheses. (E) NE-induced vena cava contraction was more sensitive than aortic contraction to TG II inhibition. (F) Densitometry of a Western analysis demonstrating the presence of TG II (70 kDa) in rat aorta and vena cava homogenates. TG II was normalized to β-actin.