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Temperature melting curve of Oris. DNA melting was measured with a Cary IE UV-visible spectrophotometer equipped with a temperature control unit. The temperature was increased or decreased at a rate of 1°C per min and held at that temperature for 30 min. The reaction curvette contained 500 nM Oris in 50 mM Tris⋅HCl (pH 8.15). The reference curvette contained the Tris buffer without Oris. The temperature ranges were 20° to 90°C (▵) and 90° to 20°C (□).
