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. 2001 Mar 6;98(6):3024–3028. doi: 10.1073/pnas.061028298

Figure 7.

Figure 7

Lack of ATP-dependent unwinding of Oris(GC)18 by UL9 protein-ICP8 complex. (a) Reaction mixtures for helicase measurements were prepared as described in Materials and Methods with 1 pmol OrisT18 or 1 pmol Oris(GC)18, and the indicated amounts of UL9 protein and ICP8. ▵, OrisT18 and Oris(GC)18, heated at 100°C for 5 min. Incubation and PAGE were performed as described in Materials and Methods. (b) Reaction mixtures for gel mobility shift measurements were prepared as described in Materials and Methods with 0.5 pmol Oris(GC)18 and the indicated amounts of UL9 protein and ICP8. Samples were processed as described in Materials and Methods. ds, double stranded; ss, single stranded.