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P1 nuclease sensitivity of Oris and OrisT18. Reaction mixtures prepared as described in Materials and Methods with 1 pmol of 32P-labeled Oris or OrisT18 and the indicated amounts of P1 nuclease and incubated at 25°C or 37°C. The products were analyzed by 10% PAGE in the presence of urea-formamide as described in Materials and Methods. M, markers.
