Table 2. Measured interstitial volumes (V_i) in naïve and anti-VEGF-administered mice.

Tissue	Vi (Literature)	Vi, Measured (Naïve)	Vi, Measured (+ B20)
brain	170–190a , d	20±5e	36±15e
spleen	200b	25±10	23±32
liver	200b , g	90±15	108±23
kidney	339b , g	2202±462f	2588±506f
intestine	174b , h	121±62	151±96
heart	143b , h	158±67	166±41
lung	300b , g	173±71	259±111
muscle	130b , i	114±119	150±47
fat	490c , i	346±259	308±121

Note: Literature values are listed for comparison. Nude mice (n = 5) were naive or administered a single, intravenous bolus dose (10 mg/kg) of the cross-species anti-VEGF antibody [12], B20-4.1, 24 h prior to assay. All values are reported in µL/g. Note that the assay method can greatly influence measured V_i values, especially in probe clearance organs.

^a [14].

^b [5].

^c [23].

^dTetramethylammonium (TMA⁺) method; corrected for a reported 9.4 µL/g blood volume [14].

^eReflects inability of ¹¹¹In-DTPA to cross the blood-brain barrier.

^fNon-physiologically relevant measurement due to renal clearance of ¹¹¹In-DTPA.

^gExtrapolated from rat; sodium (extracellular marker) content determined in a flame photometer [22].

^hEstimated on the basis of values for similar tissues [5].

ⁱExtrapolated from rat; ⁵¹Cr-EDTA method [5], [23].