Table 2. Measured interstitial volumes (Vi) in naïve and anti-VEGF-administered mice.
Tissue | Vi (Literature) | Vi, Measured (Naïve) | Vi, Measured (+ B20) |
brain | 170–190a , d | 20±5e | 36±15e |
spleen | 200b | 25±10 | 23±32 |
liver | 200b , g | 90±15 | 108±23 |
kidney | 339b , g | 2202±462f | 2588±506f |
intestine | 174b , h | 121±62 | 151±96 |
heart | 143b , h | 158±67 | 166±41 |
lung | 300b , g | 173±71 | 259±111 |
muscle | 130b , i | 114±119 | 150±47 |
fat | 490c , i | 346±259 | 308±121 |
Note: Literature values are listed for comparison. Nude mice (n = 5) were naive or administered a single, intravenous bolus dose (10 mg/kg) of the cross-species anti-VEGF antibody [12], B20-4.1, 24 h prior to assay. All values are reported in µL/g. Note that the assay method can greatly influence measured Vi values, especially in probe clearance organs.
[14].
[5].
[23].
Tetramethylammonium (TMA+) method; corrected for a reported 9.4 µL/g blood volume [14].
Reflects inability of 111In-DTPA to cross the blood-brain barrier.
Non-physiologically relevant measurement due to renal clearance of 111In-DTPA.
Extrapolated from rat; sodium (extracellular marker) content determined in a flame photometer [22].
Estimated on the basis of values for similar tissues [5].