Table 3. Volume velocities of regional blood flow (Q) in naïve and anti-VEGF-administered mice.
Tissue | Q, Literature | Q, Measured (Naïve) | Q, Measured (+ B20) |
brain | 850a , e | 12±1d | 12±1d |
spleen | 909b , f | 169±105 | 153±71 |
liver | 2103b , f | 158±20* | 200±35* |
kidney | 4881b , g | 1172±419 | 1390±537 |
intestine | 474b , f | 380±136 | 346±105 |
heart | 3828b , f | 877±63 | 1026±236 |
lung | 350a , h | 538±150 | 537±262 |
muscle | 184b , i | 186±47 | 198±59 |
fat | 190c , j | 120±37 | 142±34 |
Note: Literature values are listed for comparison. Nude mice (n = 5) were naïve or administered a single, intravenous bolus dose (10 mg/kg) of the cross-species anti-VEGF antibody [12], B20-4.1, 24 h prior to assay. All values are reported in µL/g/min. Note that the assay method can greatly influence measured values of Q, especially in probe clearance organs.
p<0.05 by unpaired t-test.
[3].
[5].
[40].
Reflects inability of probe to cross blood-brain barrier.
85Sr-labeled microspheres [15].
Origin of measurement not specified in literature.
Extrapolated from inulin clearance rates in rat kidney [41].
Bronchial flow.
Measured experimentally by Rb uptake method.
Rat data; method not specified [40].