Table 4. Sensitivity of AUC_0–7 for an antibody in mice to changes in V_v, V _i and Q.

Tissue	Model-Predicted AUC0–7 Values			Experimentala
	Lit.	Meas. (naïve)	Meas. (+ B20)	(naïve)
blood	82.5	93.0	93.1	122.2
liver	18.1	9.37	9.64	20.7
kidneys	18.7	17.8	17.1	21.8
spleen	15.4	17.7	19.4	28.1
lungs	36.7	49.5	50.2	33.1
heart	17.4	17.6	19.3	24.3
muscle	11.4	8.23	8.22	7.90
intestine	7.03	3.77	4.71	na
bone	7.58	8.55	8.56	na

Note: Comparison of model-predicted [13] and experimentally measured AUCs [%ID/g day], up to 7 days post-injection, calculated for an intact antibody in mice. All blood flow rates (Q), vascular (V_v) and interstitial (V_i) volumes were simultaneously changed from literature to measured values (see Tables 1 , 2 , 3 ) for these simulations, with the exception of V_i for kidneys, for which literature values were always used due to the effects of probe clearance. Measurement of V_v was accomplished using a previously reported indirect RBC labeling method [2].

^aData for ¹²⁵I-trastuzumab; reproduced with permissions [2].