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. 2011 Mar 17;7(3):e1001341. doi: 10.1371/journal.pgen.1001341

Figure 4. MACO-1 resides in the endoplasmic reticulum (ER).

Figure 4

Confocal optical sections of neurons in C. elegans co-stained with anti-MACO-1 and anti-GFP antibodies and expressing different markers: (A–D) the synaptic marker synaptobrevin-1-GFP (juIs1) (SNB-GFP); (E–I) an extrachromosomal array expressing the general ER marker YFP-Phosphatidylinositol synthase (YFP-PIS); (J–M) an extrachromosomal array expressing the rough ER marker Translocating chain-associating membrane protein (YFP-TRAM). Staining: A, F, J, anti-GFP antibodies; B, H, L, DAPI; C, G, K, anti-macoilin antibody. Neurons correspond to (A–D) the ventral cord motor-neurons between the gonad posterior reflex and the pre-anal ganglia, (F–I) the retrovesicular ganglia and (J–M) head. In C and D arrows indicate MACO-1 signal in the neuronal cell bodies and arrowheads indicate synapses. In I and M arrowheads indicate co-localisation between the MACO-1 and the ER markers PIS and TRAM. Scale bars represent (A–D) 2, (E) 5 and (F–M) 1 µm. N. MACO-1-mCherry fails to localize to synapses. Shown is a merge of green and red fluorescence for a section of the ventral cord from animals that express psnb-1::maco-1-mcherry (psnb-1 drives expression in all neurons) and juIs1, punc-25::snb-1-GFP. The arrows indicate cell bodies; arrowheads indicate synapses. Scale bar is 5 µm.