Skip to main content
. 2011 Mar 17;7(3):e1002017. doi: 10.1371/journal.pgen.1002017

Figure 4. lin-61 and hpl-2 act synergistically in C. elegans vulva development and fertility control.

Figure 4

(A) Representative images (DIC optics) of worms of the indicated mutant genotypes. Arrowheads point to pseudovulvae. Scale bar represents 100 µm. (B) Representative images of C. elegans of lin-61, hpl-2 and lin-61; hpl-2 mutant genotype raised at 24.5°C. DNA was stained with DAPI, Nomarski images were taken with DIC optics. The shape of gonad arms is outlined (solid lines). Dashed lines outline the embryos. White arrowheads mark nuclei of endomitotic cells implying failure in oocyte maturation and/or fertilization. Grey arrowheads mark oocytes. Asterisk marks spermatheca. Scale bar represents 50 µm. Mean brood size of non-sterile C. elegans of the indicated genotype at 20°C (C) and 24.5°C (D). Error bars reflect standard deviation. n, number of worms analyzed. One asterisk marks a significance interval of p<0.02 and three asterisks mark a significance interval of p<0.001 determined using a two-tailed Student's t-test.