Skip to main content
. 2011 Mar 17;7(3):e1001335. doi: 10.1371/journal.pgen.1001335

Figure 3. Expression of Notch, EGFR, and Smo in krz loss-of-function conditions.

Figure 3

(A–D) Expression of Notch (in red) in ap-Gal4 UAS-GFP/+; UAS-ikrz/+. A and B show two different focal planes in the ventral (A) and dorsal (B) wing disc surfaces. Below each panel are transversal sections in the ventral (“V” in A′) and dorsal (“D” in B′) disc surfaces. The bottom panels are the corresponding red channels (Notch protein expression) in these transversal sections. The white lines indicate the position of the different sections. (C) Longitudinal section of the wing blade showing the dorsal (green) and ventral (lack of GFP) surfaces showing the expression of Notch (red) Topro (blue) and GFP (green). The individual red channel is shown below. (D) Projection of 10 optical sections (indicated by a white vertical line in B′ and white horizontal line in C) spanning the apical side of an ap-Gal4 UAS-GFP/+; UAS-ikrz/+ wing disc showing Notch (red) and GFP (green) expression. Note in all cases the modest difference between wild type cells (ventral) and ikrz-expressing cells (dorsal). (E) Expression of Notch in third instar wing discs bearing clones of cells homozygous for the krz deficiency. Below each focal plane are the transversal sections showing the expression of Notch (red) and GFP (green). (E′) corresponding red channel of E. Clones were induced in hsFLP1.22/+; FRT82 Df(3R)krz/FRT82 Ubi-GFP M(3)w larvae 48–72 h. AEL (in panels E, H and J). (F) Expression of EGFR (in red), GFP (in green) and Topro (in blue) in ap-Gal4 UAS-GFP/+; UAS-ikrz/+ third instar discs. Below are transversal sections in the ventral (V, middle panel) and dorsal (D, bottom panel) disc surfaces. (F′) Red channel of F showing only EGFR expression. (F″) Longitudinal section of the wing blade showing the expression of EGFR (red) Topro (blue) and GFP (green). The individual red channel is shown below. (G) Projection of 10 optical sections spanning the apical side of a ap-Gal4 UAS-GFP/+; UAS-ikrz/+ disc showing EGFR (red) and GFP (green) expression. (H) Expression of EGFR in third instar wing discs bearing clones of cells homozygous for the krz deficiency. Below and to the right are the corresponding transversal and longitudinal sections showing the expression of EGFR (red) and GFP (green). (H′) Corresponding red channel of I. (I–I′) Expression of Smo (red) in ap-Gal4 UAS-GFP/+; UAS-ikrz/+. Below are transversal sections in the ventral (V, middle panel) and dorsal (D, bottom panel) disc surfaces. To the right are longitudinal sections in the posterior (p) and anterior (a) compartments. The individual red channels are shown in I′. (J) Expression of Smo in third instar wing discs bearing clones of cells homozygous the krz deficiency. Below each focal plane are the transversal sections showing the expression of Smo (red) and GFP (green). (J′) corresponding red channel of J. Note that in all genotypes tested the reduction or loss of krz does not modify EGFR (G–I) or Smo (J–L) expression.