Skip to main content
. 2011 Mar 17;7(3):e1001335. doi: 10.1371/journal.pgen.1001335

Figure 4. Analysis of the krz gain-of-expression phenotype and its relationship with Smo signalling.

Figure 4

(A) Wild type control wing grown at 29°C showing the positions of the longitudinal veins L2 to L5. (B–D) Weak (B), moderate (C) and strong (D) phenotypes resulting from krz over-expression in the wing blade (638-Gal4), using three different UAS-krz lines (2a2 in B, 2b2 in C and 2a1 in D). All flies were grown at 29°C. (E) Phenotype of krz over-expression in the domain of dpp expression (dpp-Gal4/UAS-krz2a1). (F–G) Strong Hh loss-of-function phenotypes obtained in smo2 homozygous wings (F; 638-Gal4/+; FRT42 smo2/FRF42 M(2)l2; UAS-FLP/+) and in Cos2 over-expressing wings (638-Gal4/+; UAS-Cos2/+; G). (H–I) Genetic interaction between hh loss of expression and krz gain of expression. The hh loss-of-function phenotype (638-Gal4/+; UAS-ihh/+; H) is strongly increased by the over-expression of Krz (638-Gal4/+; UAS-ihh/UAS-krz; I). (J–M) Expression of the Hh-target gene ptc in different Krz over-expression conditions. (J) Control third instar wing disc showing the normal expression of Ptc (red). (K) Krz over-expression in the wing blade (638-Gal4/+; UAS-krz2b2/+) reduces Ptc expression. (L) Krz over-expression in the dorsal compartment (ap-Gal4 UAS-GFP/+; UAS-krz2a3/+) reduces Ptc expression in dorsal cells (labelled in green). L′ is the red channel of L. (M) Clones of cells over-expressing Krz (labelled in green) show a cell-autonomous reduction in Ptc expression. M′ is the red channel of M. (O–Q) Expression of the Hh-target gene en in different Krz over-expression conditions. (N) Control third instar wing disc showing the normal expression of En (red). (O) Krz over-expression in the wing blade (638-Gal4/+; UAS-krz2b2/+) eliminates En expression in anterior cells (labelled by a white line in N). (P) Krz over-expression in the dorsal compartment (ap-Gal4 UAS-GFP/+; UAS-krz2a3/+) eliminates En expression in dorsal cells (labelled in green). P′ is the red channel of L. (Q) Clones of cells over-expressing Krz (labelled in green) show a cell-autonomous elimination of En expression. M′ is the red channel of M. As expected, the expression of Ptc and En is detected in wild type cells anterior to the clones of Krz over-expressing cells (M′ and Q′).