Figure 1.
Fluorimetric calcium release traces from sea urchin egg homogenates induced by the addition of increasing concentrations of NAADP and three selected NAADP analogs. Calcium-ion concentration was measured fluorimetrically using the calcium sensitive dye Fluo-3. The effect of the addition of increasing concentrations of NAADP (1)(A), 5-NH2-NAADP (20)(B), 5-CH3-NAADP (21)(C), and 4-CH3-NAADP (17)(D) on fluorescence are shown.