Figure 1. Effects of Ca²⁺, the calcimimetic NPS R-467 and the CaSR antagonist NPS2390 on eUCM-MSC growth.

Cells were plated in 6-wells plates and treated as described in materials and methods. Effects of increasing doses of extracellular Ca²⁺ on both, large (A) and small (B), cell lines. In the large cell line (A'), increasing Ca²⁺ concentration to 2.5 mM was not effective on cell growth. In the presence of 2.5 mM Ca²⁺, the allosteric activator of the CaSR, NPS R-467 (3 µM) significantly increased the cell number/well from day 7^th after treatment (P<0.001). In the small cell line (B'), increasing Ca²⁺ concentration induced cell growth on day 9^th (P<0.05). In the presence of 2.5 mM Ca²⁺, the allosteric activator of the CaSR, NPS R-467 significantly increased the cell number/well on day 9^th (P<0.05). In both cell lines, 30 min preincubation in presence of the CaSR antagonist NPS 2390 (10 µM) significantly inhibited the agonist effect. Results are mean ± SD of 2 data points. Student's t Test: a vs b: P<0.05; a vs e: P<0.01; c vs d P<0.001.