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. 2011 Mar 17;6(3):e17998. doi: 10.1371/journal.pone.0017998

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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a) Assessing overexpression of Bcl-x_L in normal cardiomyocytes and simultaneous EndoG-FLAG overexpression. b) Agarose gel electrophoresis of DNA extracts of cardiomyocytes cultured in normal conditions or after 6 hours of experimental ischemia. Cardiomyocytes were transduced 5 days before ischemia with lentivirus for overexpression of Bcl-x_L or empty viruses. HMWF and LMWF: high and low molecular weight DNA fragmentation, respectively. Similar results were obtained in three independent assays.