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. 2011 Mar 17;6(3):e17972. doi: 10.1371/journal.pone.0017972

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Tadema et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

cPR3m was produced using cPR3 plasmid-DNA provided by Dr. Preston. The protein was purified and visualized by coomassie blue staining after SDS-PAGE. The approximate molecular weight of the protein was 13 kDa. Both Rabbit-α-cPR3 (B) and Chicken-α-cPR3 (C) antibodies bound in a concentration dependant manner to cPR3m in ELISA. D) Binding of mouse-α-HIStag antibody to cPR3m in ELISA.