Figure 1. Identification of three nuclear localization sequences in an uncharacterized region of the N-terminal region of MSH6 that are only conserved in higher eukaryotes.

(A) Schematic drawing of human MSH6 detailing its N-terminal region. PCNA interaction motif (PIP) is shown in blue, PWWP domain in purple, and predicted non-specific DNA interaction domain is shown in green. Identified potential nuclear localization sequences are highlighted in blue, red and purple (NLS1, NLS2, and NLS3). Cancer mutation S285I is indicated in bold. (B) Clustal alignment of MSH6 N-terminal regions from different eukaryotic species demonstrating location of the NLSs. Yeast Msh6 contains no comparable region to those found in higher eukaryotes, as shown. The higher eukaryotic species contain highly similar and closely spaced NLSs to those found in human MSH6.