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. 2011 Mar 2;11:57. doi: 10.1186/1471-2148-11-57

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Copyright ©2011 Brideau and Barbash; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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LHR-YFP localization depends on HP1. A) Live YFP analysis of LHR-YFP in whole-mount salivary glands from third instar larvae in wild-type and HP-mutant backgrounds. A recombinant chromosome containing P{UAS-mel\Lhr::Yfp} and a salivary gland specific GAL4 driver was crossed into HP mutant backgrounds. The bright heterochromatic foci normally seen in wild type animals are absent in HP1 mutants but not in HP6 mutants. B) LHR-YFP is expressed and stable in HP mutants. Extracts from third-instar larval salivary glands were loaded on an SDS-PAGE gel and analyzed by Western blot using anti-GFP and anti-tubulin antibodies. Relative to the tubulin loading controls, LHR-YFP is present at similar levels in all samples analyzed.