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Four LHR orthologs localize to similar foci when expressed in D. melanogaster. Live YFP analysis in whole-mount salivary glands from third instar larvae expressing Lhr-Yfp from D. melanogaster, D. simulans, D. yakuba, and D. virilis. For each Lhr ortholog tested, D. melanogaster females carrying the Lhr-Yfp transgene were crossed to males carrying a salivary-gland specific GAL4 driver, and the F1 heterozygous larvae were analyzed. DNA was stained using DAPI.
