Table 2.

Testing if four Lhr orthologs have hybrid male lethal activity

	F1 Progeny Class		Number of progeny
UAS transgene in D. melanogaster mother a	Phenotype	Genotype	D. mel control cross b	D. sim Lhr1 cross c
P{UAS-mel\Lhr::Yfp}1	Red-eyed male	UAS/+; GAL4/+ and	278	134
		+/+; GAL4/+
	Orange-eyed male	UAS/+; +/+	148	107
	White-eyed male	+/+; +/+	143	141
		Total	569 n.s.	382***
P{UAS-mel\Lhr::Yfp}2	Red-eyed male	UAS/+; GAL4/+ and	221	334
		+/+; GAL4/+
	Orange-eyed male	UAS/+; +/+	93	8
	White-eyed male	+/+; +/+	102	314
		Total	416 n.s.	656***
P{UAS-mel\Lhr::Yfp}3	Red-eyed male	UAS/+; GAL4/+ and	282	74
		+/+; GAL4/+
	Orange-eyed male	UAS/+; +/+	162	2
	White-eyed male	+/+; +/+	175	82
		Total	619 n.s.	158***
Φ {UAS-sim\Lhr::Yfp}	Red-eyed male	UAS/+; GAL4/+ and	139	117
		+/+; GAL4/+
	Orange-eyed male	UAS/+; +/+	72	39
	White-eyed male	+/+; +/+	77	107
		Total	288 n.s.	223***
Φ {UAS-yak\Lhr::Yfp}	Red-eyed male	UAS/+; GAL4/+ and	83	346
		+/+; GAL4/+
	Orange-eyed male	UAS/+; +/+	37	55
	White-eyed male	+/+; +/+	45	396
		Total	165 n.s.	797***
Φ {UAS-vir\Lhr::Yfp}	Red-eyed male	UAS/+; GAL4/+ and	158	164
		+/+; GAL4/+
	Orange-eyed male	UAS/+; +/+	76	114
	White-eyed male	+/+; +/+	86	112
		Total	320 n.s.	390 n.s.

^a - Females heterozygous for the indicated UAS transgene and for P{Act5C-GAL4}17bFO1 were crossed to either D. melanogaster w¹¹¹⁸/Y (control cross) or to D. simulans Lhr¹ males. For the first two sets of crosses the females were of genotype w/w; UAS/+; GAL4/+; for the remaining crosses the females were of genotype w/w; UAS +/GAL4 +.

^b - For the control crosses the ratios of red eye: orange eye: white eye progeny were tested for deviation from a 2:1:1 ratio using a chi-square test. n.s. = p > 0.05

^c - The ratios of red eye: orange eye: white eye for hybrid crosses were compared to control crosses using a 3 × 2 contingency table and chi-square test. *** = p < 1 × 10^-3; n.s. = p > 0.05, 2 degrees of freedom.