Table 4.
Summary of binding kinetics measured using Octet RED96
| Immobilised biotinylated Pex5p(C) |
Ligand | Association rate, kon (1/Ms) |
Dissociation rate, koff (1/s) |
|---|---|---|---|
| Wild-type | mSCP2 | 2.3 × 104 (± 2.0 × 102) | 5.5 × 10-5 (± 2.0 × 10-6) |
| Wild-type | preSCP2 | 3.5 × 104 (± 2.8 × 102) | 7.9 × 10-5 (± 1.5 × 10-6) |
| S600W | mSCP2 | 6.5 × 103 (± 3.9 × 101) | 6.0 × 10-5 (± 1.7 × 10-6) |
| S600W | preSCP2 | 7.3 × 103 (± 4.8 × 101) | 5.1 × 10-5 (± 1.8 × 10-6) |
| R608W | mSCP2 | 7.2 × 103 (± 4.8 × 101) | 7.3 × 10-5 (± 1.8 × 10-6) |
| R608W | preSCP2 | 9.0 × 103 (± 6.4 × 101) | 5.0 × 10-5 (± 1.9 × 10-6) |
| D624W | mSCP2 | 1.1 × 104 (± 8.3 × 101) | 1.5 × 10-4 (± 2.3 × 10-6) |
| D624W | preSCP2 | 1.3 × 104 (± 1.1 × 102) | 8.6 × 10-5 (± 2.2 × 10-6) |