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. 2011 Feb 8;12:7. doi: 10.1186/1471-2091-12-7

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Copyright ©2011 Stakkestad et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expressed RIα and RIIα inhibit expressed Cα1 and Cβ2 catalytic activity in a dose-dependent manner. 293T cells were co-transfected with increasing amounts (0-1280 ng) of either pDeRIα (A and B) or pExRIIα (C and D) with a fixed amount of pDeCα1 (300 ng, A and C) or pDeCβ2 (1400 ng, B and D). Cells were harvested after 24 hours, cell extracts adjusted to 1 mg total protein/mL and assayed for PKA-specific phosphotransferase activity in the presence (+ cAMP) and absence (- cAMP) of 7.14 μM cAMP. Data points represent pmol ATP transferred/min/mg protein) +/- SD (n = 2-6).