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. 2011 Mar 4;4:31. doi: 10.1186/1756-3305-4-31

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Copyright ©2011 Salim et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Agarose gel electrophoresis of field T. evansi DNA samples (50 ng) amplified with species specific primers TeRoTat920Fand TeRoTat1070R for T. evansi based on RoTat 1.2 VSG gene. A: Lane M1: 6 band marker. All other lanes are RoTat 1.2 VSG gene positive (8, 9, 12 and 17 weak positives; 8 & 9 from South Darfur); except lane 3, 4 and 16 were shown RoTate 1.2 VSG negative from Khowai. Samples shown here are from geographically different areas. B: Lane M1 6 band marker. Lanes 2-15 have camel samples. 7 and 9 are weak positives, 12 and 14 are strong positives, rest are negative, ie. 2-6, 8, 10, 11, 13 and 15. Lane 16 and 17 are control negative and positive respectively. (All samples were from Darfur). Extra bands in both gels are non-specific bands.