Figure 5. C. glabrata is responsive to host intracellular labile Fe pools.

A J774A.1 cells expressing the mutant FPN transgenes were infected with the C. glabrata SIT1mCherry strain reporter and visualized upon co-culture for 8 hours using live microscopy. Fluorescein-conjugated dextran beads were added prior to co-culture to ascertain whether yeast cells were located intracellularly rather than on the surface of macrophages. B Macrophage Fe overload is associated with increased C. glabrata Fe satiety whereas the severe macrophage Fe depletion associated with Fpn^C326Y leads to elevated SIT1 expression. C. glabrata cells were recovered from infected macrophages at the indicated time points and the levels of SIT1 mRNA quantified by RT-PCR. Each experiment included 3 replicates per experimental sample. Bars represent the standard error.