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. 2011 Mar 17;7(3):e1001318. doi: 10.1371/journal.ppat.1001318

Figure 5. Antigen is directly presented to effector cells by hepatocytes.

Figure 5

A. C57Bl/6 or TAP1-/- mice received 2×106 SIINFEKL-specific effector CD8+ T cells 3 hours prior to challenge with 5×103 P. berghei CS5M sporozoites (grey bars); control mice did not receive effector cells (black bars). 40 hours later livers were taken and parasite rRNA concentration determined by real-time PCR (mean ± SEM; n = 4, data from one of 2 similar experiments). B. C57Bl/6 or 3d mice received 2×106 SIINFEKL specific effector CD8+ T cells 3 hours prior to challenge with 5×103 P. berghei CS5M sporozoites (grey bars); control mice did not receive effector cells (black bars). 40 hours later livers were taken and parasite rRNA concentration determined by real-time PCR (mean ± SEM; n = 5, data from one of 2 similar experiments). C. Cyt c or PBS treated C57Bl/6 mice received 2×106 SIINFEKL specific effector CD8+ T cells 3 hours prior to challenge with 5×103 P. berghei CS5M sporozoites (grey bars); control mice did not receive effector cells (black bars). Treated mice received 15 mg cyt c for 3 days starting the day before challenge. 40 hours after challenge livers were taken and parasite rRNA concentration determined by real-time PCR (mean ± SEM; n = 4).