Fig. 4.

CDX1 is sufficient and required for lumen formation even in hypoxia but not for goblet cell differentiation. (A–C) Unlike the HCT116 vector control (HCT116-vec), HCT116-CDX1 forms lumens in both normoxia and hypoxia. Knockdown of CDX1 in LS174T (LS174T-siCDX1) reduces the proportion of large colonies that can form lumens compared with the vector control (LS174T-vec; D) but has no significant effect on the proportion of mucin expressing goblet cells. (E and F) Goblet cell mucin (PR4D4) is green. Nuclei are stained blue with DAPI. (Magnification: 20× objective.) Fisher's exact tests were significant in B, C, and D, but not E. (Scale bar: 200 μm.)