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Characterization of zebrafish lkb1 mutations. (A) Whole-mount in situ hybridizations show decreased lkb1 mRNA expression in 3-dpf lkb1-mutant larvae. (B) Western blot analysis of AMPK and ACC upon KCN treatment. KCN treatment results in activation of the AMPK pathway in 3-dpf WT larvae but not in 3-dpf lkb1-mutant larvae.
