Fig. 5.

(A) This plot shows a typical experiment where a molecule was extended (blue trace) and relaxed (red trace) two times and then it was overextended in order to detach it from the surface (black trace). This last trace has a single detachment force peak consistent with the stretching pattern of a single molecule. (B) The forward and reverse traces are well characterized by the WLC equation (dotted line) and there is no hysteresis in the time scale of the experiment (∼500 ms). (C) Histogram for the persistence length shows a mean of 0.35 ± 0.09 nm (n = 147; 26 molecules).