TABLE 5.
Steady-state kinetic parameters for pol β deoxyribose lyase activity
| Enzyme | Kcat (min−1) | Km (nM) | Kcat/Km (nM−1 min−1) |
|---|---|---|---|
| WT | 4.1×10−3 | 262 | 1.6×10−5 |
| p.K27N | 1.1×10−3 | 77 | 1.4×10−5 |
Note: the kinetic values presented are calculated in the absence of APE I, as detailed in Materials and Methods.