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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Cell Physiol. 2011 Jun;226(6):1499–1509. doi: 10.1002/jcp.22479

Figure 3.

Figure 3

Lung myofibroblasts synthesize both Ltbp-3 and Ltbp-4. P0.5 WT lung sections were analyzed by immunofluorescence using antibodies against SMC/myofibroblast specific αSMA and an antibody against either Ltbp-4 (A) or Ltbp-3 (B). Nuclei were stained with DAPI. A. Double immunofluorescence of WT cells stained with both Ltbp-4 (left panel) and αSMA (middle panel). The yellow signal indicates overlap of Ltbp-4 and αSMA signals (right panel, arrowheads). B. Double immunofluorescence of WT cells stained with both Ltbp-3 (left panel) and αSMA (middle panel). A small but real number ofαSMA-positive cells (arrows) was also stained with Ltbp-3 (arrowheads), indicating that Ltbp-3 is expressed in some lung myofibroblasts. Bars: 20μm.