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(A) Gel-mobility shift assay of the binding of purified His6-tagged ribosomal protein S1 to 100 pM of 32P-labeled SsrA RNA produced by transcription in vitro. Arrows mark the positions of the bound and free RNA species. (B) Binding curve generated from data in A. (C) Binding of His6-tagged ribosomal protein S1 to 100 pM 32P-labeled PK1L and PK3L SsrA variants.
