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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Hypertension. 2011 Feb 28;57(4):764–771. doi: 10.1161/HYPERTENSIONAHA.110.165803

Figure 4. Expression analysis of Rififylin and Levels of polyubiquitinated proteins.

Figure 4

(a) Expression of Rffl transcript at 53 days of age as detected by RT-PCR. (b) Quantification of Rffl transcripts by Real time PCR (n=6 animals/group). (c) Immunoblot of Rffl in whole-cell lysates from S (n=3) and congenic (n=3) rat hearts at 53 days of age. 36.41kDa, RFFL (NP_0010717368, 2aa–99aa) partial recombinant protein was used as positive control and β-Actin was the loading control. Quantification of Rffl protein± SEM is shown on the right. **p<0.01. (d) Immunoblot for polyubiquitinated proteins in whole-cell lysates from S (n=2) and congenic (n=3) rat hearts at 110 days of age. Control: provided by the Pierce Ubiquitination enrichment kit. Quantification of these proteins ± SEM is shown in the right. **p<0.01.