Fig. 4.

Comparison of anti-gp140 specific antibodies and total immunoglobulins in vaginal and cervical secretions. Animals were immunised either once or three times by intramuscular immunisation with gp-140 in AS01 adjuvant followed by a cycle of 9 intravaginal immunisations with gp140 in Carbopol gel (Groups B and C) (a) The median titres of IgG and IgA antibodies were significantly different at the P = 0.006 (**) level for IgG but were not significantly different (ns) for IgA P = 0.097 (Mann–Whitney ranked sum test). (b) Total IgG concentrations were significantly higher in cervical secretions, P = 0.001 (**) (Mann–Whitney ranked sum test). (c) Total IgA concentrations in fluids from the two sites were not significantly different, P = 0.22. Group mean (- - -) and median (—) titres are shown with box plots.