Table 3.

Serum virus neutralising activity.

Animal No.	Day 0 (pre-imm.)				Day 196 (26–47 days post 3× ivag)				Day 281 (111–132 days post 3× ivag)				Day 315 (34 days post 1× i.m.)
	VNA		Binding Ab		VNA		Binding Ab		VNA		Binding Ab		VNA		Binding Ab
	Clade C (MW965)	Clade B (SF162)	IgG	IgA	Clade C (MW965)	Clade B (SF162)	IgG	IgA	Clade C (MW965)	Clade B (SF162)	IgG	IgA	Clade C (MW965)	Clade B (SF162)	IgG	IgA
(a) Group A
E53	<60	<60	<100	<10	NT	NT	<100	<10	<60	<60	<100	<10	<60	<60	40,800	1370
E54	<60	<60	<100	<10	<60	<60	7600	940	<60	<60	3100	710	1980	151	134,000	16,000
E55	<60	<60	<100	<10	<60	<60	1200	120	288a	74a	<100	70	72	<60	82,900	6300
E56	<60	<60	<100	<10	NT	NT	<100	<10	<60	<60	<100	<10	<60	<60	16,000	800

Animal No.	Day 0 (pre-imm.)				Day 64 (64 days post 1× i.m.)				Day 99–115 (9–14 days post 1× ivag)				Day 199 (98–110 days post 1× ivag)
	VNA		Binding Ab		VNA		Binding Ab		VNA		Binding Ab		VNA		Binding Ab
	Clade C (MW965)	Clade B (SF162)	IgG	IgA	Clade C (MW965)	Clade B (SF162)	IgG	IgA	Clade C (MW965)	Clade B (SF162)	IgG	IgA	Clade C (MW965)	Clade B (SF162)	IgG	IgA
(b) Group B
E49	<60	<60	<100	<10	<60	<60	1470	1810	65	<60	1200	720	<60	<60	450	190
E50	<60	<60	<100	<10	<60	<60	690	91	74	<60	2260	635	<60	<60	700	140
E51	<60	<60	<100	<10	<60	<60	420	45	115	<60	2130	980	<60	<60	400	200
E52	<60	<60	<100	<10	<60	<60	1330	156	<60	<60	2840	900	<60	<60	540	190

Animal No.	Day 0 (pre-imm.)				Day 98 (14 days post 3× i.m.)				Day 134–148 (36–50 days post 3× i.m.)				Day 160–179 (13–22 days post 1× ivag)				Day 270–272 (111–127 days post 1× ivag)
	VNA		Binding Ab		VNA		Binding Ab		VNA		Binding Ab		VNA		Binding Ab		VNA		Binding Ab
	Clade C (MW965)	Clade B (SF162)	IgG	IgA	Clade C (MW965)	Clade B (SF162)	IgG	IgA	Clade C (MW965)	Clade B (SF162)	IgG	IgA	Clade C (MW965)	Clade B (Sf162)	IgG	IgA	Clade C (MW965)	Clade B (SF162)	IgG	IgA
(c) Group C
E57	<60	<60	<100	<10	1814	453	12,4400	8700	594	121	22,700	2900	159	<60	NT	NT	196	<60	7600	590
E58	<60	<60	<100	<10	2128	<60	112,400	6400	761	<60	21,500	2560	517	<60	15,200	1950	192	<60	4300	400
E59	<60	<60	<100	<10	860	73	71,200	6400	216	<60	9360	1100	105	<60	4300	510	<60	<60	2000	140
E60	<60	<60	<100	<10	2846	96	127,600	11,200	1012	69	23,800	1880	651	<60	13,000	1550	125	<60	2100	190

Animal No.	Day 0 (pre imm.)				Day 34 (34 days post i.m.)
	VNA		Binding Ab		VNA		Binding Ab
	Clade C (MW965)	Clade B (SF162)	IgG	IgA	Clade C (MW965)	Clade B (SF162)	IgG	IgA
(d) Group D
E107	<60	<60	<100	<10	<60	<60	2000	120
E108	<60	<60	<100	<10	<60	<60	1600	190

Bold values indicate specific neutralisation with no background activity on MuLV pseudotype virus.

^aNon-specific activity – VNA titre of 640 against MuLV pseudotype virus.