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. 2010 Dec 20;589(Pt 4):863–875. doi: 10.1113/jphysiol.2010.200808

Figure 6. Induction of ROS assessed by flow cytometry using a lower RA concentration for a longer treatment time.

Figure 6

HESC were incubated for 6 h in RA (1 μm), TGF-β (5 ng ml−1), RA + TGF-β, or vehicle control (Con) as indicated and analysed for fluorescence in DCF-loaded cells by flow cytometry as described in Methods. Results are expressed as mean fluorescence intensity (MFI) and percentage positive cells relative to an isotype control antibody.