Skip to main content
. Author manuscript; available in PMC: 2012 Feb 11.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Jan 8;405(2):256–261. doi: 10.1016/j.bbrc.2011.01.021

Fig. 4.

Fig. 4

Psoralen-induced osteoblast differentiation is BMP-2- and BMP-4-dependent. Primary calvarial osteoblasts isolated from 3-day-old neonatal Bmp2fx/fx;Bmp4fx/fx mice were infected with either Ad-GFP or Ad-Cre and then treated with or without 100 µM Psoralen for 2 days. A) qPCR data indicated that infection of Ad-Cre efficiently deleted the Bmp2 and Bmp4 gene in primary osteoblasts. B–D) Deletion of the Bmp2 and Bmp4 genes partially reversed Psoralen-induced expression of Col1, Alp and Bsp in osteoblasts. *p<0.05 when compared to the group without Psoralen.