Figure 7.

Transfer of particle-associated viral RNAs into HK-2 cells. Quantitative RT-PCR for two spliced and three unspliced forms of HIV RNAs was used to evaluate the viral RNA species transferred into HK-2 cells at 3 and 24 hours after co-culture with infected T cells. (A) Viral RNA profile from virion-associated RNAs from pelleted HIV mixed with uninfected HK-2 cells. (B) Viral RNAs from 1% mixture of HIV infected Jurkat cells and HK-2 cells. (C) Viral RNAs from cells sorted after 3 hours. (D) Viral RNAs from cells sorted after 24 hours. (E) Quantitative RT PCR for CD3 genes (CD3 ε and γ chain) to determine the level of T-cell contamination in the 3- and 24-hour sorted cell populations.