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. 2011 Mar;22(3):518–525. doi: 10.1681/ASN.2010060661

Figure 4.

Figure 4.

Rf-1+3+4_b kidneys have increased protein casting and glomerular sclerosis compared with Rf-1+4 and Rf-1+3+4_a kidneys. (A) Histologic sections (10×) of (1) Rf-1 + 4,(2) Rf-1 + 3+4_a, and (3) Rf-1 + 3+4_b medulla and histologic sections (20×) of (4) Rf-1 + 4,(5) Rf-1 + 3+4_a, and (6) Rf-1 + 3+4_b cortex. All kidney sections were stained using Gomori's one-step trichrome stain. Rf-1 + 3+4_b kidneys showed larger and more abundant protein casts (stained red) compared with Rf-1 + 4 and Rf-1 + 3+4_a kidneys (panels 1 to 3). Panel 6 shows glomerular sclerosis (+) and interstitial fibrosis (→) in Rf-1 + 3+4_b cortex. (B) Quantification of percent area of protein casting in the outer stripe of the medulla and cortex for Rf-1 + 4 (n = 4), Rf-1 + 3+4_a (n = 3), and Rf-1 + 3+4_b (n = 4). (C) Average sclerosis score for Rf-1 + 4 (n = 4), Rf-1 + 3+4_a (n = 3), and Rf-1 + 3+4_b (n = 4) glomeruli. **P < 0.01; ***P < 0.001. Statistical comparisons were done using a one-way ANOVA followed by a Holm-Sidak post hoc test.