FIGURE 5.

CaV1.2 exons 8a and 8 shift their splicing during development of embryonic mouse cortex. A, exon 8 progressively decreased in inclusion as exon 8a increased from embryonic days 12 (E12) to 18 (E18). The PCR products and sizes are indicated on the left. Note that transcripts excluding both exons 8a and 8 (ΔExon) were also observed in embryonic cortex. From 7 to 10 embryonic cortices were pooled for RNA and protein extraction at each time point. B, immunoblot of proteins in developing cortex shows decreasing PTB protein expression between embryonic days 12 and 18, whereas nPTB expression moderately increased, as normalized to GAPDH expression.